|
KMID : 0364820070430030201
|
|
Korean Journal of Microbiology
2007 Volume.43 No. 3 p.201 ~ p.209
|
|
|
Phylogenetic Characteristics of Viable but Nonculturable Bacterial Populations in a Pine Mushroom (Tricholoma matsutake) Forest Soil
|
|
Kim Yun-Ji
Whang Kyung-Sook
|
|
|
Abstract
|
|
|
The CFDA (6-carboxyfluorescein diacetate) direct viable count method and plate count (PC) method using conventional
nutrient broth (NB) medium and 10-2 diluted NB (DNB) medium were applied to samples collected from Mt. Yongdoo in Andong, in an effort to determine the number of living bacteria pine mushroom forest soil. The number of living bacteria determined via plate count in NB medium comprised 5~8% of the CFDA direct viable count, and the bacteria in the DNB medium comprised 40~47%. This result indicated that viable but nonculturable (VBNC) bacteria existed in the pine mushroom forest soil at a high percentage. The phylogenetic characteristics of the VBNC bacterial populations in the samples of pine mushroom (Tricholoma matsutake) forest soil were analyzed via the direct extraction of DNA and 16S rDNA-ARDRA. The 115 clones from pine mushroom forest soil were clustered into 31 different RFLP phylotypes by ARDRA. Based on the 16S rDNA sequences, the 31 ARDRA clusters were classified into 6 phylogenetic groups: α-, β-, γ-Proteobacteria, Acidobacteria, Actinobacteria and Firmicutes. Among these bacterial populations, approximately 85% were classified as members of phylum Acidobacteria. The Acidobacteria phylum was shown to exist abundantly in the pine mushroom forest soil.
|
|
|
KEYWORD
|
|
|
Acidobacteria, forest soil, phylogeny, pine mushroom, VBNC bacterial population
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|